05) values for mostly all sperm kinetics. Sperm kinetics showed that TL_A exhibited high (p <. 05) in post-thaw motility and recovery rate (38.33 ± 6.41%, 62.76 ± 8.10%) compared to centrifuged samples TL_A_ Cent.
Caffeine supplemented centrifuged samples (TL_AC_ Cent.) showed superiority ( P <.
Using 4 mM caffeine in amylase-treated semen (TL_AC) improved post-thaw motility 50.0 ± 1.29% and recovery rate 77.8 ± 3.83% compared to the control (40.17 ± 2.79% and 62.55 ± 8.39%), respectively. The second experiment investigated the effect of caffeine addition to tolerate enzymatic and mechanical stress. group showed significant viscosity reduction (0.33 ± 0.05) and motility decline 47.85 ± 3.04% with increment in abnormalities and detached acrosome. After equilibration, control group recorded 62.85 ± 4.28% motility and 1.3 ± 0.13 viscosity score, while TL_A group values were 72.88 ± 3.30% and 0.83 ± 0.07%, respectively.
MINITUBE BOAR SEMEN FREEZING FREE
Tris lactose supplemented with amylase followed by seminal plasma removal via centrifugation (TL_A_ Cent.) and re-suspension into enzyme free TL media. Tris lactose supplemented with amylase (TL_A) and III. In the first experiment, three groups, namely: I. Two experiments were carried out to determine efficiency of centrifugation in the presence of a mucolytic agent for liquefaction of dromedary semen.
Kenney (often marketed under a brand name - e.g.Assisted reproductive technologies have been reported to improve reproductive efficiency and genetic potential in camelids. 
MS Dilufert, various generations (3, 6, 9, 10). When private donors ship chilled semen outside of the formal regulatory environment, and fertilization is accomplished by allowing sperm to swim through the reproductive tract without the help of procedures such as IVF, then it is possible to achieve better results without antibiotics. This is because in procedures such as IVF with frozen sperm, the sperm does not need to swim up the reproductive tract on their own, and the detrimental effects of the antibiotics are not problematic. In human semen extenders, antibiotics are required for regulatory reasons, so their use is almost universal in clinics, even though antibiotics can be detrimental to sperm. The latter - gentamicin - has been noted to reduce sperm motility in the equine. Ticarcillin (often used in combination with clavulanic acid under the designation timentin), amikacin sulfate, penicillin, and gentamicin are commonly used. Other extenders (e.g., INRA '96) may also contain milk components.Īntibiotics are almost universal in semen extenders, especially those being used for shipping or freezing semen. Dual-sugar extenders typically have similar ingredients, with an additional sugar, sucrose. Kenney) has been used for many years, and contains a non-fat dried milk solid (NFDMS) and glucose. In the equine Kenney extender (named after its developer, Dr. Egg yolk, which has cryoprotective properties, is also a common component. Typical cryoprotectants include glycerol, DMSO and dimethylformamide. In the case of freezing extenders, one or more penetrating cryoprotectants will be added. Semen extender also serves to protect sperm from bacteria by adding antibiotics to it to prevent increase of bacteria. The addition of extender to semen protects the sperm cells against possible damage by toxic seminal plasma, as well as providing nutrients and cooling buffers if the semen is to be cooled.
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